Fig. 1: Microfluidic device design and specifications. | Microsystems & Nanoengineering

Fig. 1: Microfluidic device design and specifications.

From: Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis

Fig. 1

The microfluidic device is designed for assessing M. smegmatis cell trapping, growth, and drug treatment for continuous live kinetic growth studies. This device is composed of arrays of M. smegmatis trapping/culture chambers that are only 0.9 μm in height (yellow box) and connected through arrays of deeper (14 μm in height) perfusion channels (cyan color). A polydimethyl siloxane (PDMS) microchannel layer is bonded onto a thin cover glass to form the microfluidic device.

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