Fig. 4: Fabrication of single-cell-laden hydrogels. | Microsystems & Nanoengineering

Fig. 4: Fabrication of single-cell-laden hydrogels.

From: Oil-mediated high-throughput generation and sorting of water-in-water droplets

Fig. 4

a Schematic showing the process of crosslinking the alginate solution by Ca2+ diffusion. b Image of the formed monodisperse alginate microgels. Scale bar: 200 μm. c Design of the integrated channel to perform continuous droplet generation and sorting. d Image illustrating the sorting process: The droplets with single cells are driven to the positive channel, while the droplets without cells flow into the negative channel. Scale bar: 100 μm. e Comparison of the formed hydrogels without and with sorting after a Live/Dead assay. Scale bar: 100 μm. f A plot of the percentages of the droplets encapsulating zero, single and multiple cells without and with sorting

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