Fig. 5: Results of F-actin alignment and distributions of three typical proteins in the cell–cell junction areas. | Microsystems & Nanoengineering

Fig. 5: Results of F-actin alignment and distributions of three typical proteins in the cell–cell junction areas.

From: A micropore array-based solid lift-off method for highly efficient and controllable cell alignment and spreading

Fig. 5

Typical immunofluorescence images of single-cell (a), double-cell (b), and triple-cell (c) alignments on micropatterns with different arrangements/distributions (vertical, square, and lateral) and the extracted distributions of F-actin alignments obtained via MATLAB processing. Immunofluorescence images of vinculin (d), Cx43 (e), and N-cadherin (f) (double-cell alignments in vertical, square, and lateral arrangements). Distributions of vinculin (g), Cx43 (h), and N-cadherin (i) in the cell–cell junction areas, analyzed using ImageJ and MATLAB processing

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