Fig. 2: Protein (Alexa Fluor 488-conjugated FN) patterning with the solid lift-off method. | Microsystems & Nanoengineering

Fig. 2: Protein (Alexa Fluor 488-conjugated FN) patterning with the solid lift-off method.

From: A micropore array-based solid lift-off method for highly efficient and controllable cell alignment and spreading

Fig. 2

a Protein micropatterns obtained with different surface treatments of substrates and incubation time lengths of FN solution. b Differences between the designed and experimentally obtained sizes of protein micropatterns by the present solid lift-off method and previously reported techniques23,26,49,50,51,52,53. c Typical scanning electron microscopy (SEM) images of the Parylene C micropore arrays (shadow masks) and fluorescence images of the corresponding protein micropatterns of various shapes and sizes on flat substrates. d Schematic illustration of protein micropattern preparation on curved substrates via the solid lift-off method. e PDMS tubes without and with micropore arrays adhered on the inside walls, and the SEM image of the used micropore arrays. f Typical confocal images of the achieved protein micropatterns on the curved substrates (i.e., the inner wall surface of PDMS tubes with radius of curvature at 0.5 mm), in views of different angles. #These values were calculated according to the figures in the original references

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