Fig. 7: Detection of 5-HT from RIN14B cells cultured in a T75 flask. | Microsystems & Nanoengineering

Fig. 7: Detection of 5-HT from RIN14B cells cultured in a T75 flask.

From: Electrochemical measurement of serotonin by Au-CNT electrodes fabricated on microporous cell culture membranes

Fig. 7

a Illustration of cell supernatant transferred from the T75 flask cell culture to the Au-CNT (2 µL) electrode-integrated membrane platform for CV measurement. b, c CV measurement of cell supernatant before (−) and after (+) stimulation with butyrate. b Representative CV curves. c Epa and Ipa values obtained from all curves in (b) (n = 5 cycles per measurement). d Electrode calibration with 5-HT standards in DMEM. e Linear fit of 5-HT standards (n = 3 cycles per measurement). Ipa values measured from the supernatant before and after butyrate stimulation are fit on the line to approximate 5-HT concentration in each sample: ~3.1 µM before (−) butyrate, ~4.1 µM after (+) butyrate (excluding outlier). A 5-min accumulation time was used for all CV measurements

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