Fig. 4: Optimization results of the assay reagents. | Microsystems & Nanoengineering

Fig. 4: Optimization results of the assay reagents.

From: A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria

Fig. 4

a 96 well plate assay results plotted on the right axis and Chemifluorescence assay result obtained from Optimizer™ microfluidic microplate is plotted on the left axis showing lower LOD and higher dynamic range and b Chemifluorescence signal output variation with increase in PfHRP2 capture antibody (CAb) concentration (bottom axis) and increase in PfHRP2 detection antibody (DAb-HRP) concentration (top axis) at a fixed DAb-HRP concentration (0.1 μg/mL) and at a fixed CAb concentration (100.0 μg/mL), respectively. Each point represents the mean of three replicates

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