Fig. 2: Immunoassay protocol and working principle of the MCFA chip. | Microsystems & Nanoengineering

Fig. 2: Immunoassay protocol and working principle of the MCFA chip.

From: A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria

Fig. 2

a Schematic of the immunoassay protocol for chemiluminescence based sandwich ELISA as implemented on the lab chip, b Schematic of the immunoassay reactions occurring in the test reaction chamber, positive control chamber and negative control chamber (from left to right) and c Sequential working principle of the MCFA device where (i) shows the empty test chamber immobilized with capture antibody, (ii) shows the condition when HRP-DAb-antigen complex reaches the test chamber, (iii) depicts the formation of Cab-antigen-DAb complex after adequate washing and (iv) illustrates the condition when the substrate reaches the test chamber producing chemiluminescence

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