Fig. 5: Performance of HiFi-SIM on reconstructing raw data with low modulation depth and strong background. | Light: Science & Applications

Fig. 5: Performance of HiFi-SIM on reconstructing raw data with low modulation depth and strong background.

From: High-fidelity structured illumination microscopy by point-spread-function engineering

Fig. 5

a Equivalent wide-field image of microtubules in live U2OS cells with strong background fluorescence is shown in the left, and the SR images reconstructed using fairSIM and HiFi-SIM. Lower-right corner shows corresponding reconstructed spectrum. bd Magnified images of the red-box, cyan-box, and magenta-box regions in a. eg Intensity profiles along the red, cyan, and magenta lines in bd, respectively

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