Fig. 4: Performance of HiFi-SIM on reconstructing raw data with high-quality. | Light: Science & Applications

Fig. 4: Performance of HiFi-SIM on reconstructing raw data with high-quality.

From: High-fidelity structured illumination microscopy by point-spread-function engineering

Fig. 4

a TIRF wide-field image of microtubules in live COS-7 cells is shown in the left, and the SR images of fairSIM and HiFi-SIM are presented in the middle and right, respectively. Lower-right corner shows corresponding reconstructed spectrum. b Magnified images of the white-box region in a. c Intensity profiles along the red line in b. d Equivalent wide-field image of microtubules in fixed COS-7 cells is shown in the left triangle, and the SR images were reconstructed using GE SoftWoRx, Hessian-SIM, and HiFi-SIM, respectively. e, f Magnified images of the wide-field equivalent in the white-box and red-box regions in d are shown in the left, and the corresponding SR images were reconstructed by SoftWoRx, Hessian-SIM, and HiFi-SIM, respectively. Here, Hessian-SIM uses the same OTF as used in fairSIM (Fig. S11), and uses the built-in notch filters to suppress pattern artifacts (a0 = 0.05, v = 1.2)

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