Fig. 4: Optical manipulation of a single fluorescent nanoparticle. | Light: Science & Applications

Fig. 4: Optical manipulation of a single fluorescent nanoparticle.

From: Single-cell biomagnifier for optical nanoscopes and nanotweezers

Fig. 4

a Schematic diagram showing a fluorescent nanoparticle suspended on the surface of a mirror and trapped by the biomagnifier. b SEM image showing the PS fluorescent nanoparticles with an average radius of 50 nm. c Emission spectrum showing the central emission wavelength of the fluorescent nanoparticles located at 600 nm. d–f Optical images show the trapping process of a single PS nanoparticle with the biomagnifier. The process consisted of three successive steps: before trapping (d), during trapping (e), and after release (f). gi Fluorescence images showing the fluorescence spot of the PS nanoparticle before being trapped (g), during trapping (h), and after release (i). jl Three-dimensional color mapping of the fluorescence spots of the nanoparticle as shown in gi. m Real-time trace of the position of the trapped nanoparticle in the x and y directions. n Trapping potential of the trapped nanoparticle in the x and y directions with parabola fittings. o Composite fluorescence images show the movement trace of the trapped nanoparticle in the x–y plane by controlled movement of the biomagnifier

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