Fig. 5: Resolving the 3D architecture of the cellular microtubule network with mirror-enhanced dSTORM. | Light: Science & Applications

Fig. 5: Resolving the 3D architecture of the cellular microtubule network with mirror-enhanced dSTORM.

From: Sharpening emitter localization in front of a tuned mirror

Fig. 5

a, b Scatter plot reconstructions of Cos7 microtubule networks immunolabeled with A647 and imaged with dSTORM in a the SSD configuration and b the mirror-enhanced configuration. The color code illustrates the median of the localization uncertainty of all events localized in the respective pixel area. c, d Magnified image sections from b. The white arrows indicate microtubule crossings where different axial positions of the filaments are distinguishable based on the localization uncertainty. Scale bars: 2 µm (a, b) and 500 nm (c, d)

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