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Characterization of leukemia progression in the Cbfb-MYH11 knockin mice by single cell RNA sequencing

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Fig. 1: Comparison of single cell transcriptome of bone marrow Kit+ progenitor/stem cells in wild-type mice and in conditional Cbfb-MYH11 knockin mice at pre-leukemic and leukemic stages.
Fig. 2: Identification of leukemia precursor cells and their candidate markers in the pre-leukemia mice.

Data availability

The datasets generated during and/or analyzed during the current study are available in the Gene Expression Omnibus (GEO) repository,


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The work was supported by the Intramural Research Programs of the National Human Genome Research Institute, National Institute on Deafness and Other Communication Disorders, and Natioal Cancer Institute, NIH. The authors would like to thank Zhi Liu and Jim Mullikin for preliminary STREAM testing, Erika Kwon, Erica Bresciani and Elisabeth Heuston for insightful discussion, as well as Luigi Alvarado (now at 10X Genomics) and the LaRochelle lab at NHLBI (NIH) for critical pilot drop-seq experiments that led to this study. This work used the computational resources of the NIH highperformance computing Biowulf cluster (

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JD performed experiments, analyzed data, and wrote the manuscript, KY analyzed data and prepared several figures for the manuscript, MK assisted in sequencing, read mapping, and downsampling for sequencing depth normalization, TZ and GL prepared the conditional knockin mice used, and SA performed FACS isolation of the cells of interest.

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Correspondence to Paul Liu.

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Diemer, J.L., Yu, K., Kelly, M. et al. Characterization of leukemia progression in the Cbfb-MYH11 knockin mice by single cell RNA sequencing. Leukemia 37, 1549–1553 (2023).

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