Clinicohematologic and molecular response of essential thrombocythemia patients treated with pegylated interferon-α: a multi-center study of the German Study Group-Myeloproliferative Neoplasms (GSG-MPN)

Clinicohematologic and molecular response of essential thrombocythemia patients treated with pegylated interferon-α: a multi-center study of the German Study Group-Myeloproliferative Neoplasms (GSG-MPN) Frank Stegelmann 1,9, Lino L. Teichmann 2,3,9, Florian H. Heidel, Carl C. Crodel, Thomas Ernst , Sebastian Kreil, Andreas Reiter, Sara Otten, Stefanie Schauer, Ruth-Miriam Körber, Kim Kricheldorf, Susanne Isfort, Hartmut Döhner , Tim H. Brümmendorf , Martin Griesshammer, Konstanze Döhner 1 and Steffen Koschmieder 3,7

Treatment with hydroxyurea (HU) or anagrelide (ANA) aims at normalizing the platelet (PLT) count and decreasing the risk of vascular complications. However, long-term intake of HU is associated with multiple side effects leading to discontinuation in half of the patients [3]. Furthermore, HU and ANA do not modify the natural course of ET, whereas interferon-α (IFN) may also prevent disease progression to SMF, as recently shown for polycythemia vera (PV) [4,5]. This notion is supported by the observation of molecular responses (MR) in a subset of ET patients (partial MR [PMR], i.e., ≥50% allele burden reduction, in 15-57% and complete MR [CMR] in up to 33% of patients) [6][7][8][9].
Pegylated formulations of IFN (pegIFN) reduce treatmentrelated adverse events and allow for the extension of the application intervals. However, the accessibility to pegIFN is currently limited to PV patients due to a lack of approval in ET.
In a recent meta-analysis of 30 ET studies conducted between 1990 and 2014, data on the use of IFN were promising, including complete hematologic response rates of 59% and an annual discontinuation rate due to adverse events of 9% [9]. Although this analysis included a total number of 730 ET patients, there are critical limitations: (i) the majority of patients (490/730, 67%) were treated with non-pegylated IFN, (ii) the median number of patients per study was low (n = 20), and (iii) the time of follow-up was limited (median 24 months for pegIFN studies, range 9-83).
To investigate the clinical benefits of pegIFN in ET, we here report the results from a retrospective analysis of a large cohort of 127 ET patients treated in routine clinical practice at seven academic centers. All patients provided written informed consent to the German Study Group-Myeloproliferative Neoplasms (GSG-MPN) registry (NCT03125707). ET was diagnosed after bone marrow biopsy in all cases based on the latest WHO classification at that time.
The median age of patients at ET diagnosis was 37.0 years (range, 8.2-77.4); 67% were female and 33% were male (Table 1). Median baseline PLT count and median white blood cell (WBC) count at the start of pegIFN was 780/nL (124-2776) and 8.2/nL (3.0-17.3), respectively. The presence of immature white or red precursor cells in the peripheral blood was excluded in all patients. Furthermore, 51% of the patients were JAK2 V617F, 32% CALR, and 5% MPL mutated. According to the International Prognostic Score of thrombosis in ET (IPSET-thrombosis), 37% were low-, 31% intermediate-, and 30% high-risk (2% unknown) [10]. Splenomegaly, as assessed by clinical palpation or ultrasound, was present in 29% of the patients (median diameter by ultrasound 11.05 cm).
According to the high-risk definition of the European Leuke-miaNet (ELN) cytoreductive treatment was indicated in patients at age >60 years, with a history of thrombosis or major bleeding, and/or PLT count >1500/nL [11]. Median time from ET diagnosis to start of pegIFN treatment was 1.9 years (0.0-37.2). Notably, 59% of the patients received pegIFN as first-line treatment, 20% were pretreated with HU, 9% with ANA, and 11% with both; one patient had received busulfan prior to pegIFN. Main reasons for starting pegIFN treatment in our study were extreme thrombocytosis in 39%, prior thrombosis in 31%, persistent ET-associated symptoms (e.g., microcirculatory disturbances) in 9%, age >60 years in 7%, and pregnancy in 7% (change from other cytoreductive treatment) of cases.
According to ELN response criteria, 89% of patients achieved clinicohematologic response at any time point during pegIFN treatment, which was complete in 54% and partial in 35% of patients (9% no response, 2% unknown) [12]. The median PLT and WBC count at the last follow-up (380/nL and 5.5/nL) and at the best response (369/nL and 5.1/nL) were considerably lower compared to assessment before pegIFN administration (780/nL and 8.2/nL) (Fig. 1C). Data on pegIFN dosing as well as on the time point of best response were not available.
Notably, just one disease progression to SMF and no transformation to sAML occurred in 469 pegIFN treatment years. Major vascular complications were also rare, with three arterial and five venous events resulting in an incidence of 0.6% and 1.1% per patient and year, respectively. Of interest, all arterial events were thromboembolic myocardial infarctions, whereas 4/5 venous complications were abdominal vein thromboses (one pulmonary embolism). Flu-like symptoms occurred in 41%, elevated liver function tests in 16% and depression in 14% of LOT (grading and time points of events not assessed).
Furthermore, we calculated odds ratios for the achievement of complete clinicohematologic response with respect to prior cytoreductive therapy, IPSET-thrombosis risk category, JAK2 mutation status, and sex (Fig. 1D): Although significance was not reached, these analyses show a favorable trend for patients without prior cytoreduction, at high-risk for vascular complications, and with the presence of a JAK2 V617F mutation.
In addition, we performed digital droplet PCR measurements with a sensitivity of 10 −3 to quantify the JAK2 V617F variant allele frequency (VAF) before and during pegIFN treatment [13]. In total, 70 quantifications were performed in 14 patients with the availability of DNA from peripheral blood granulocytes at three time points or more: the first sample within 1 year prior to pegIFN   (Fig. 1E). There were no statistically significant differences compared to baseline. Of note, 5/14 patients (36%) showed PMR at the last follow-up compared to baseline. All patients with PMR had a baseline VAF >10% (13.6-48.0), which decreased to 8.9% (median, range 0.99-19.4) at the last follow-up, and median time to ≥50% JAK2 V617F VAF reduction was 4 years (2)(3)(4)(5). Only one of the responders showed a VAF <1% (0.99%) at the last follow-up.
In 10/127 patients (8%), pegIFN-2b was stopped due to market withdrawal. Based on preliminary data showing that treatmentfree remissions may be achieved in a subset of long-term pegIFNtreated patients, they were not immediately switched to an alternative drug as they had been treated for a median of 9.5 years (6-18) [14]. While 7/10 patients re-started pegIFN (or an alternative cytoreductive therapy) after a median treatment-free time of 7 months (3-15) due to an increased platelet count, 3/10 patients treated over 15-18 years maintained normal blood cell counts until data cut-off (1.5-4.5 years after discontinuation). Interestingly, 4/7 patients with loss of complete hematologic response were JAK2 V617F positive and all four patients showed an allele burden of >5% at the time of pegIFN discontinuation: 5.6%, 16.7%, 19.9%, and 41.7% (the remaining three patients were CALR mutated; VAF not available). In contrast, 2/3 of patients maintaining complete hematologic response had undetectable JAK2 V617F (<0.1%), while 1/3 was at 0.1% at pegIFN stop in 2017. In this patient, JAK2 V617F rose slowly to 0.54% after 4 years of follow-up without medication although PLT count remained normal. These three patients were not included in Fig. 1E since no DNA prior to pegIFN treatment (2001)(2002) was available for quantification of JAK2 V617F allelic burden. Bone marrow biopsies were performed in 2/3 of patients who maintained normal blood cell counts over 1.5 and 4.5 years after pegIFN discontinuation, respectively. It is instructive that, in both cases, histomorphologic remission of ET was demonstrated.
In summary, our data on 127 WHO-defined ET patients treated in routine clinical practice confirms the high efficacy of pegIFN in achieving a clinicohematologic response. Our study stands out for its unique sample size, a very long follow-up time of a subset of patients, and shows low rates of adverse events, vascular complications, and disease progressions. In the absence of randomized clinical trials and approval, our data support the use of pegIFN in ET patients. It is important to note that in individual cases it still remains difficult to distinguish ET from prefibrotic/ early primary MF (prePMF). Therefore, one cannot fully exclude the possibility that some patients in our study had prePMF.
Prospective studies assessing JAK2 allele burden monitoring and defining thresholds for planned treatment discontinuation in PV patients treated with ropegIFN are currently ongoing, and the results will be instructive for the management of ET as well [15]. In contrast to PV, quantification of mutated CALR remains an additional challenge in ET treated with pegIFN.