A Samples analyzed with scRNA + TCRab-seq are marked on the clinical timeline. Blood counts are plotted in upper four panels (B-Hb = hemoglobin (g/l), B-neut = neutrophils (E9/l), B-trom = thrombocytes (E9/l), E-retik = reticulocyte percentage of erythrocytes). Corticosteroid (prednisolone) and cyclosporine A (CyA) dosages (mg) are plotted in two panels below. Timing of ATG treatments is marked with arrows. B In AA-3, TCRBV04-03 clone was expanding from sample 1 (diagnosis) to relapse (sample 3). On the x axis are different time points and y axis shows the frequency of each TCRb clone from whole CTL TCRb repertoire. C Amplicon sequencing confirmed all mutations in Vbeta 7.2+ (corresponding to TCRBV04-03) fraction of CD8+ T cells. y axis shows variant allele frequencies within Vbeta 7.2+ CD8+ T cells at different time points (shown at x axis). D Possible subclonal architecture of the mutations.