A Density plot of variant allele frequencies of CD4+ and CD8+ specific variants detected with immunogene panel sequencing. B Fraction-specific somatic mutation burden in AA and healthy samples. Mann–Whitney U test was used in pairwise comparisons within each disease group and cell fraction, only statistically significant (p < 0.05) differences are marked. C Mutation burden in CD8+ T cells was associated with T-cell clonality (Spearman ρ = 0.44, p = 0.012). Each group is shown separately on the left panel (Spearman test values for AA: ρ = 0.71, p = 0.012; healthy: ρ = 0.21, p = 0.37). D Number of somatic mutations in CD8+ T cells did not have significant correlation with age at sample collection, although a positive trend was observed.