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Acute myeloid leukemia

Notch blockade overcomes endothelial cell-mediated resistance of FLT3/ITD-positive AML progenitors to AC220 treatment

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Fig. 1: EC coculture improved survival of FLT3/ITD-positive progenitors treated with AC220.
Fig. 2: Inhibiting Notch signaling reduced EC-mediated protection of FLT3/ITD-positive CFCs in the presence of AC220 for patient samples with high FLT3-AR.

Data availability

RNA-seq data are available at GEO, accession GSE138340.

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Acknowledgements

This study was supported by The Hartwell Fellowship Foundation to QL. We thank Chris Siebel (Genentech) for the anti-NRR1 and anti-NRR2 antibodies and Shahin Rafii (Weill Cornell Medical College) for the E4ORF1 lentivirus construct.

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QL and IB designed the experiments; QL performed the experiments and analyzed the data; QL, IB, BH, and SM wrote the paper.

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Correspondence to Irwin Bernstein.

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Le, Q., Hadland, B., Meshinchi, S. et al. Notch blockade overcomes endothelial cell-mediated resistance of FLT3/ITD-positive AML progenitors to AC220 treatment. Leukemia 35, 601–605 (2021). https://doi.org/10.1038/s41375-020-0893-y

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