a Schematic diagram of CD93 expression within a stem cell population. b CD93 gene expression as determined in the lin−CD34+CD38−CD90+CD93+ and lin−CD34+CD38−CD90+CD93− population (p = 0.0068). Results are presented as fold change compared with the CD34+ bulk population using the ΔΔCt method. c Key genes involved in lineage restriction were overexpressed in the lin−CD34+CD38−CD90+CD93− population, namely GATA1 and CBX8. d Key genes involved in stem cell maintenance were upregulated in the lin−CD34+CD38−CD90+CD93+ population. e Normalized differential gene expression of the single cells using the ΔΔCt method. The control sample is the mean of the CD93− on each chip. GATA1 was significantly downregulated in the CD93+ population (n = 2 CP-CML samples, q = 0.03). f Principal component analysis (PCA) of the four different populations defined by CD93 surface presence and BCR-ABL1 expression. Each individual dot represents a single cell. It can be observed that the CD93+ BCR-ABL1+ cells (in red) are more heterogeneous than the other populations and that they form a slightly different cluster.