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Long non-coding RNA DLGAP1-AS1 modulates the development of non-small-cell lung cancer via the microRNA-193a-5p/DTL axis


Non-small cell lung cancer (NSCLC) is one of the most malignant cancers worldwide. A growing number of studies have suggested that long noncoding RNAs (lncRNAs) play a key role in the progression of non-small cell lung cancer (NSCLC). Here, we report a novel lncRNA DLGAP1 antisense RNA 1 (DLGAP1-AS1) that exhibits oncogenic properties in NSCLC. The lncRNA DLGAP1-AS1 and denticleless protein homolog (DTL) presented upregulated expression, but microRNA-193a-5p (miR-193a-5p) showed downregulated expression in cancerous tissues of human lung samples from 48 patients with NSCLC. Partial loss of lncRNA DLGAP1-AS1 reduced malignant cell viability, migration, and invasion but induced apoptosis. Dual-luciferase reporter gene, RNA pull-down and RNA binding protein immunoprecipitation assays demonstrated enrichment of lncRNA DLGAP1-AS1 in miR-193a-5p and Argonaute 2, suggesting that lncRNA DLGAP1-AS1 modulated DTL, a putative target of miR-193a-5p. We also found that restoration of miR-193a-5p rescued NSCLC cell biological functions affected by overexpression of lncRNA DLGAP1-AS1. Silencing lncRNA DLGAP1-AS1 was found to reduce the tumorigenesis of NSCLC cells xenografted into nude mice, which was rescued by DTL overexpression. In conclusion, our study highlights a novel regulatory network of the lncRNA DLGAP1-AS1/miR-193a-5p/DTL axis in NSCLC, providing a potential therapeutic strategy for NSCLC.

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Fig. 1: Upregulated lncRNA DLGAP1-AS1 is found in NSCLC tissues and cells.
Fig. 2: Silencing of lncRNA DLGAP1-AS1 restricts NCl-H1975 cell viability, migration, and invasion but induces apoptosis in NCl-H1975 cells.
Fig. 3: Elevated expression of miR-193a-5p suppresses NSCLC cell viability, migration, and invasion, but induces apoptosis.
Fig. 4: LncRNA DLGAP1-AS1 upregulates DTL by binding to miR-193a-5p.
Fig. 5: Silencing lncRNA DLGAP1-AS1 suppresses NSCLC cell viability, migration, and invasion, but accelerates apoptosis via the miR-193a-5p/DTL axis.
Fig. 6: Silencing of lncRNA DLGAP1-AS1 inhibits NSCLC tumor growth in vivo via the miR-193a-5p/DTL axis.

Data availability

The datasets generated/analysed during the current study are available.


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This work is supported by National Natural Science Foundation of China (No. 31101641) and the project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations



Xudong Pan conceived and designed research. Siwen Chen performed experiments and interpreted results of experiments. Lu Ye analyzed data. Shenjie Xu prepared figures. Ling Wang drafted paper. Yi Sun edited and revised manuscript. All authors read and approved final version of manuscript.

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Correspondence to Ling Wang or Yi Sun.

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The authors declare no competing interests.

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The study was performed with the approval of the Ethics Committee of The First Affiliated Hospital of Soochow University. Each patient provided informed written consent prior to study recruitment. All experiments in the present study were conducted in strict accordance with the Helsinki Declaration. The animal experiment strictly adhered to the principle to minimize the pain, suffering, and discomfort to experimental animals. The protocol was approved by the Animal Ethics Committee of The First Affiliated Hospital of Soochow University.

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Pan, X., Chen, S., Ye, L. et al. Long non-coding RNA DLGAP1-AS1 modulates the development of non-small-cell lung cancer via the microRNA-193a-5p/DTL axis. Lab Invest 102, 1182–1191 (2022).

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