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Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations

Laboratory Investigationvolume 99pages93106 (2019) | Download Citation

Abstract

The lack of consensus on bone marrow (BM) and splenic immune cell profiles in preclinical mouse strains complicates comparative analysis across different studies. Although studies have documented relative distribution of immune cells from peripheral blood in mice, similar studies for BM and spleen from naïve mice are lacking. In an effort to establish strain- and gender-specific benchmarks for distribution of various immune cell subtypes in these organs, we performed immunophenotypic analysis of BM cells and splenocytes from both genders of three commonly used murine strains (C57BL/6NCr, 129/SvHsd, and BALB/cAnNCr). Total neutrophils and splenic macrophages were significantly higher in C57BL/6NCr, whereas total B cells were lower. Within C57BL/6NCr female mice, BM B cells were elevated with respect to the males whereas splenic mDCs and splenic neutrophils were reduced. Within BALB/cAnNCr male mice, BM CD4+ Tregs were elevated with respect to the other strains. Furthermore, in male BALB/cAnNCr mice, NK cells were elevated with respect to the other strains in both BM and spleen. Splenic CD4+ Tregs and splenic CD8+ T cells were reduced in male BALB/c mice in comparison to female mice. Bone marrow CD4+ T cells and mDCs were significantly increased in 129/SvHsd whereas splenic CD8+ T cells were reduced. In general, males exhibited higher immature myeloid cells, macrophages, and NK cells. To our knowledge, this study provides a first attempt to systematically establish organ-specific benchmarks on immune cells in studies involving these mouse strains.

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Acknowledgements

Financial support from the National Institute of Health research grants CA184770 and AR060948, and the Department of Defense grant PR141945, is greatly acknowledged. We sincerely thank ENVIGO for providing mice for the study.

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  1. These authors contributed equally: Jonathan A. Hensel, Vinayak Khattar

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  1. Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA

    • Jonathan A. Hensel
    • , Vinayak Khattar
    • , Reading Ashton
    •  & Selvarangan Ponnazhagan

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Correspondence to Selvarangan Ponnazhagan.

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https://doi.org/10.1038/s41374-018-0137-1