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Dectin-1 stimulates IL-33 expression in dendritic cells via upregulation of IRF4

Laboratory Investigationvolume 98pages708714 (2018) | Download Citation


Interleukin-33 (IL-33) is a potent contributor to antiviral immune responses and antitumor immunity. We recently discovered that IL-33 is overexpressed in dectin-1-activated dendritic cells (DCs). However, mechanisms of dectin-1-induced IL-33 expression in DCs remain elusive. Curdlan, an agonist of dectin-1, was used to mature DCs in this study. We found that dectin-1-induced IL-33 expression in DCs relies on Syk and Raf-1 pathways. By using nuclear factor (NF)-κB inhibitors, we also found that dectin-1-induced IL-33 expression relies on NF-κB signaling. Furthermore, through Syk/Raf-1-NF-κB pathway, dectin-1 signaling stimulates DCs to overexpress interferon regulatory factor 4 (IRF4), which directly upregulates the expression of IL-33 in dectin-1-activated DCs. Thus, our study provides new insights into the mechanisms of dectin-1-induced IL-33 expression in DCs and may provide new targets for improving DC-based cancer immunotherapy.

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This work was supported by grants from National Natural Science Foundation of China (no. 81372536 for SW, 81502452 for XC and 81602485 for YZ) and a grant from the Natural Science Foundation of Jilin Province (no. 20170520006JH for XC).

Author information


  1. Department of Gynecological Oncology, The First Hospital of Jilin University, Changchun, 130061, China

    • Dongjiao Wang
    • , Ning Liu
    •  & Ying Yue
  2. Department of Hematology, The First Hospital of Jilin University, Changchun, 130061, China

    • Sujun Gao
    •  & Tianxue Qin
  3. Department of Cancer Immunology, The First Hospital of Jilin University, Changchun, 130061, China

    • Jintong Chen
    • , Yinghua Zhao
    • , Yuxue Jiang
    • , Xiao Chu
    • , Qing Yi
    •  & Siqing Wang
  4. Department of Internal Medicine, Linhai First People’s Hospital, Linhai, Zhejiang, 317000, China

    • Xiaohua Wang
  5. Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, 44195, USA

    • Qing Yi


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The authors declare that they have no conflict of interest.

Corresponding authors

Correspondence to Ying Yue or Siqing Wang.

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