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Leukocyte matrix metalloproteinase and tissue inhibitor gene expression patterns in children with primary hypertension

Journal of Human Hypertension volume 34, pages 355–363 (2020)Cite this article

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Abstract

Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play an important role in cardiovascular remodeling. The aim of the study was to analyze MMP/TIMP genes expression in peripheral blood leukocytes of 80 hypertensive children (15.1 ± 2.0 years) in comparison with age-matched 78 normotensive children (14.6 ± 2.0 years; n.s.). TIMP and MMP expression in peripheral blood leukocytes was assessed by quantitative real-time PCR. Hypertensive children independently of age, sex, and body mass index had greater expression of MMP-2 than normotensive controls (p = 0.0001). Patients with left ventricular hypertrophy had greater expression of MMP-14 than patients with normal left ventricular mass (p = 0.006) and TIMP-2 expression correlated with carotid wall cross-sectional area (p = 0.03; r = 0.238). MMP-14 expression correlated with BMI-SDS (p = 0.001; r = 0.371), waist circumference-SDS (p = 0.016; r = 0.290), hsCRP (p = 0.003; r = 0.350), serum HDL-cholesterol (p = 0.008; r = −0.304), and serum uric acid (p = 0.0001; r = 0.394). In conclusion, hypertensive adolescents presented significant alterations of MMP/TIMP expression pattern in comparison with normotensive peers. Moreover, altered MMP/TIMP expression was associated with hypertensive target organ damage and metabolic abnormalities.

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Source(s) of funding

The study was supported by a grant funded by National Center of Research (2011/01/B/NZ6/02661).

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Author notes

  1. These authors contributed equally: Joanna B. Trojanek, Anna Niemirska

  2. These authors jointly supervised this work: Jacek Michałkiewicz, Mieczysław Litwin

Authors and Affiliations

  1. Department of Microbiology and Clinical Immunology, The Children’s Memorial Health Institute, Warsaw, Poland

    Joanna B. Trojanek, Renata Grzywa & Jacek Michałkiewicz

  2. Department of Nephrology and Arterial Hypertension, The Children’s Memorial Health Institute, Warsaw, Poland

    Anna Niemirska, Łukasz Obrycki & Mieczysław Litwin

  3. Department of Biochemistry & Experimental Medicine, The Children’s Memorial Health Institute, Warsaw, Poland

    Aldona Wierzbicka

  4. Department of Public Health, The Children’s Memorial Health Institute, Warsaw, Poland

    Zbigniew Kułaga

  5. Department of Endocrinology and Diabetology, The Children’s Memorial Health Institute, Warsaw, Poland

    Mieczysław Szalecki

Authors
  1. Joanna B. Trojanek
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Trojanek, J.B., Niemirska, A., Grzywa, R. et al. Leukocyte matrix metalloproteinase and tissue inhibitor gene expression patterns in children with primary hypertension. J Hum Hypertens 34, 355–363 (2020). https://doi.org/10.1038/s41371-019-0197-8

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