Use of personal care products, such as makeup, soaps, and sunscreen, may expose adolescent girls to potential endocrine disruptors, including phthalates, parabens, and other phenols. We evaluated the relationship between recent self-reported personal care product use and concentrations for urinary metabolites of phthalates, parabens, triclosan, and benzophenone-3 (BP-3) in 100 Latina adolescents. Girls who reported using makeup every day vs. rarely/never had higher urinary concentrations of monoethyl phthalate (MEP) (102.2 ng/mL vs. 52.4 ng/mL, P-value: 0.04), methyl paraben (MP) (120.5 ng/mL vs. 13.4 ng/mL, P-value < 0.01), and propyl paraben (PP) (60.4 ng/mL vs. 2.9 ng/mL, P-value < 0.01). Girls who reported recent use of specific makeup products, including foundation, blush, and mascara, had higher urinary concentrations of MEP, mono-n-butyl phthalate (MBP), MP, and PP. Use of Colgate Total toothpaste was associated with 86.7% higher urinary triclosan concentrations. Use of sunscreen was associated with 57.8% higher urinary concentrations of BP-3. Our findings suggest that personal care product use is associated with higher exposure to certain phthalates, parabens, and other phenols in urine. This may be especially relevant in adolescent girls who have high use of personal care products during a period of important reproductive development.
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Kelley KE, Hernández-Díaz S, Chaplin EL, Hauser RB, Mitchell AA. Identification of phthalates in medications and dietary supplement formulations in the United States and Canada. 2011.
Koniecki D, Wang R, Moody RP, Zhu J. Phthalates in cosmetic and personal care products: concentrations and possible dermal exposure. Environ Res. 2011;111:329–36.
Schecter A, Lorber M, Guo Y, Wu Q, Yun SH, Kannan K, et al. Phthalate concentrations and dietary exposure from food purchased in New York State. Environ Health Perspect . 2013;121:473.
Guo Y, Kannan K. A survey of phthalates and parabens in personal care products from the United States and its implications for human exposure. Environ Sci Technol. 2013;47:14442–9.
Program NT. Butylparaben [CAS No. 94-26-8] Review of toxicological literature. 2005.
Food U, Administration D. Safety and effectiveness of consumer antiseptics; topical antimicrobial drug products for over-the-counter human use; proposed amendment of the tentative final monograph; reopening of administrative record. 2013.
Dann AB, Hontela A. Triclosan: environmental exposure, toxicity and mechanisms of action. J Appl Toxicol. 2011;31:285–311.
Health Nlo. Household products database. Look up CAS. 2007.
Rastogi SC. UV filters in sunscreen products−a survey. Contact Dermat. 2002;46:348–51.
Suzuki T, Kitamura S, Khota R, Sugihara K, Fujimoto N, Ohta S. Estrogenic and antiandrogenic activities of 17 benzophenone derivatives used as UV stabilizers and sunscreens. Toxicol Appl Pharmacol. 2005;203:9–17.
Krause M, Klit A, Blomberg Jensen M, Søeborg T, Frederiksen H, Schlumpf M, et al. Sunscreens: are they beneficial for health? An overview of endocrine disrupting properties of UV‐filters. Int J Androl. 2012;35:424–36.
Shen O, Du G, Sun H, Wu W, Jiang Y, Song L, et al. Comparison of in vitro hormone activities of selected phthalates using reporter gene assays. Toxicol Lett. 2009;191:9–14.
Karpuzoglu E, Holladay SD, Gogal RM Jr. Parabens: potential impact of low-affinity estrogen receptor binding chemicals on human health. J Toxicol Environ Health Part B. 2013;16:321–35.
Witorsch RJ. Critical analysis of endocrine disruptive activity of triclosan and its relevance to human exposure through the use of personal care products. Crit Rev Toxicol. 2014;44:535–55.
Organization WH. Diethyl phthalate. Concise international chemical assessment document 52. Geneva, Switzerland: World Health Organization; 2003. http://www.inchem.org/documents/cicads/cicads/cicad52.htm
Zorrilla LM, Gibson EK, Jeffay SC, Crofton KM, Setzer WR, Cooper RL, et al. The effects of triclosan on puberty and thyroid hormones in male Wistar rats. Toxicol Sci. 2009;107:56–64.
Mylchreest E, Sar M, Cattley RC, Foster PM. Disruption of androgen-regulated male reproductive development by di (n-butyl) phthalate during late gestation in rats is different from flutamide. Toxicol Appl Pharmacol. 1999;156:81–95.
Gray LE, Ostby J, Furr J, Price M, Veeramachaneni DR, Parks L. Perinatal exposure to the phthalates DEHP, BBP, and DINP, but not DEP, DMP, or DOTP, alters sexual differentiation of the male rat. Toxicol Sci. 2000;58:350–65.
Foster P. Disruption of reproductive development in male rat offspring following in utero exposure to phthalate esters. Int J Androl. 2006;29:140–7.
Jönsson BA, Richthoff J, Rylander L, Giwercman A, Hagmar L. Urinary phthalate metabolites and biomarkers of reproductive function in young men. Epidemiology. 2005;16:487–93.
Miodovnik A, Engel SM, Zhu C, Ye X, Soorya LV, Silva MJ, et al. Endocrine disruptors and childhood social impairment. Neurotoxicology. 2011;32:261–7.
Colón I, Caro D, Bourdony CJ, Rosario O. Identification of phthalate esters in the serum of young Puerto Rican girls with premature breast development. Environ Health Perspect. 2000;108:895.
Schoeters G, Den Hond E, Dhooge W, Van Larebeke N, Leijs M. Endocrine disruptors and abnormalities of pubertal development. Basic Clin Pharmacol Toxicol. 2008;102:168–75.
Ormond G, Nieuwenhuijsen MJ, Nelson P, Toledano MB, Iszatt N, Geneletti S, et al. Endocrine disruptors in the workplace, hair spray, folate supplementation, and risk of hypospadias: case-control study. Environ Health Perspect. 2009;117:303.
Jacobson-Dickman E, Lee MM. The influence of endocrine disruptors on pubertal timing. Curr Opin Endocrinol Diabetes Obes. 2009;16:25–30.
Wolff MS, Teitelbaum SL, Pinney SM, Windham G, Liao L, Biro F, et al. Investigation of relationships between urinary biomarkers of phytoestrogens, phthalates, and phenols and pubertal stages in girls. Environ Health Perspect. 2010;118:1039–46.
Mustafa M, Bakhiet M, Wondimu B, Modeer T. Effect of triclosan on interferon‐γ production and major histocompatibility complex class II expression in human gingival fibroblasts. J Clin Periodontol. 2000;27:733–7.
Xu H, Shao X, Zhang Z, Zou Y, Wu X, Yang L. Oxidative stress and immune related gene expression following exposure to di-n-butyl phthalate and diethyl phthalate in zebrafish embryos. Ecotoxicol Environ Saf. 2013;93:39–44.
Anderson SE, Franko J, Kashon ML, Anderson KL, Hubbs AF, Lukomska E, et al. Exposure to triclosan augments the allergic response to ovalbumin in a mouse model of asthma. Toxicol Sci. 2012;132:13.
Anderson SE, Meade BJ, Long CM, Lukomska E, Marshall NB Investigations of immunotoxicity and allergic potential induced by topical application of triclosan in mice. J Immunotoxicol. 2015;13:1–8.
Marshall NB, Lukomska E, Long CM, Kashon ML, Sharpnack DD, Nayak AP, et al. Triclosan induces thymic stromal lymphopoietin in skin promoting Th2 allergic responses. Toxicol Sci. 2015;147:127–39.
Kwon J-T, Yang Y-S, Kang M-S, Seo G-B, Lee DH, Yang M-J, et al. Pulmonary toxicity screening of triclosan in rats after intratracheal instillation. J Toxicol Sci. 2013;38:471–5.
Kato T, Tada‐Oikawa S, Takahashi K, Saito K, Wang L, Nishio A, et al. Endocrine disruptors that deplete glutathione levels in APC promote Th2 polarization in mice leading to the exacerbation of airway inflammation. Eur J Immunol. 2006;36:1199–209.
Ait Bamai Y, Shibata E, Saito I, Araki A, Kanazawa A, Morimoto K, et al. Exposure to house dust phthalates in relation to asthma and allergies in both children and adults. Sci Total Environ. 2014;485-486:153–63.
Beko G, Callesen M, Weschler CJ, Toftum J, Langer S, Sigsgaard T, et al. Phthalate exposure through different pathways and allergic sensitization in preschool children with asthma, allergic rhinoconjunctivitis and atopic dermatitis. Environ Res. 2015;137:432–9.
Savage JH, Matsui EC, Wood RA, Keet CA. Urinary levels of triclosan and parabens are associated with aeroallergen and food sensitization. Journal Allergy Clin Immunol. 2012;130:453–60.
Spanier AJ, Fausnight T, Camacho TF, Braun JM (eds). The associations of triclosan and paraben exposure with allergen sensitization and wheeze in children. Allergy and Asthma Proceedings. OceanSide Publications, Inc. East Providence, Rhode Island 2014.
Clayton R, Erin M, Todd M, Dowd JB, Aiello AE. The impact of bisphenol a and triclosan on immune parameters in the U. S. population, NHANES 2003 a 2006. Environ Health Perspect. 2010;119:390–6.
Bertelsen RJ, Carlsen KC, Calafat AM, Hoppin JA, Haland G, Mowinckel P, et al. Urinary biomarkers for phthalates associated with asthma in Norwegian children. Environ Health Perspect. 2013;121:251–6.
Bertelsen RJ, Longnecker MP, Løvik M, Calafat AM, Carlsen KH, London SJ, et al. Triclosan exposure and allergic sensitization in Norwegian children. Allergy. 2013;68:84–91.
Ku HY, Su PH, Wen HJ, Sun HL, Wang CJ, Chen HY, et al. Prenatal and postnatal exposure to phthalate esters and asthma: a 9-year follow-up study of a taiwanese birth cohort. PLoS ONE. 2015;10:e0123309.
Lee H-R, Hwang K-A, Nam K-H, Kim H-C, Choi K-C. Progression of breast cancer cells was enhanced by endocrine-disrupting chemicals, triclosan and octylphenol, via an estrogen receptor-dependent signaling pathway in cellular and mouse xenograft models. Chem Res Toxicol. 2014;27:834–42.
Olaniyan L, Mkwetshana N, Okoh A. Triclosan in water, implications for human and environmental health. SpringerPlus . 2016;5:1639.
Paul KB, Hedge JM, Bansal R, Zoeller RT, Peter R, DeVito MJ, et al. Developmental triclosan exposure decreases maternal, fetal, and early neonatal thyroxine: a dynamic and kinetic evaluation of a putative mode-of-action. Toxicology. 2012;300:31–45.
Geens T, Dirtu AC, Dirinck E, Malarvannan G, Van Gaal L, Jorens PG, et al. Daily intake of bisphenol A and triclosan and their association with anthropometric data, thyroid hormones and weight loss in overweight and obese individuals. Environ Int. 2015;76:98–105.
Bedoux G, Roig B, Thomas O, Dupont V, Le Bot B. Occurrence and toxicity of antimicrobial triclosan and by-products in the environment. Environ Sci Pollut Res. 2012;19:1044–65.
Fang J-L, Stingley RL, Beland FA, Harrouk W, Lumpkins DL, Howard P. Occurrence, efficacy, metabolism, and toxicity of triclosan. J Environ Sci Health, Part C. 2010;28:147–71.
Zhou Y, Wang H, Chen Y, Jiang Q. Environmental and food contamination with plasticisers in China. Lancet. 2011;378:e4.
Bazin I, Gadal A, Touraud E, Roig B. Hydroxy benzoate preservatives (parabens) in the environment: data for environmental toxicity assessment. Xenobiotics in the urban water cycle: Springer. 2010; pp 245–57.
Kim S, Choi K. Occurrences, toxicities, and ecological risks of benzophenone-3, a common component of organic sunscreen products: a mini-review. Environ Int. 2014;70:143–57.
Rudel RA, Camann DE, Spengler JD, Korn LR, Brody JG. Phthalates, alkylphenols, pesticides, polybrominated diphenyl ethers, and other endocrine-disrupting compounds in indoor air and dust. Environ Sci Technol. 2003;37:4543-4553.
Cirillo T, Fasano E, Esposito F, Prete ED, Cocchieri RA. Study on the influence of temperature, storage time and packaging type on di-n-butylphthalate and di (2-ethylhexyl) phthalate release into packed meals. Food Addit Contam. 2013;30:403–11.
Zota AR, Calafat AM, Woodruff TJ. Temporal trends in phthalate exposures: findings from the National Health and Nutrition Examination Survey, 2001-2010. Environ Health Perspect. 2014;122:235–41.
Calafat AM, Ye X, Wong LY, Bishop AM, Needham LL. Urinary concentrations of four parabens in the U.S. population: NHANES 2005-2006. Environ Health Perspect. 2010;118:679–85.
Han C, Lim YH, Hong YC. Ten-year trends in urinary concentrations of triclosan and benzophenone-3 in the general U.S. population from 2003 to 2012. Environ Pollut. 2016;208:803–10.
Centers for Disease Control and Prevention. Fourth Report on Human Exposure to Environmental Chemicals, Updated Tables, February 2015. Atlanta, GA: U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. http://www.cdc.gov/exposurereport/. 2015.
Environmental Working Group. Teen girls’ body burden of hormone-altering cosmetics chemicals: Environmental Working Group. http://www.ewg.org/book/export/html/26953. 2008.
CDC. National Health and Nutrition Examination Survey Data. US Department of Health and Human Services. 2013–2014.
Parlett LE, Calafat AM, Swan SH. Women’s exposure to phthalates in relation to use of personal care products. J Expo Sci Environ Epidemiol. 2013;23:197–206.
Buckley JP, Palmieri RT, Matuszewski JM, Herring AH, Baird DD, Hartmann KE, et al. Consumer product exposures associated with urinary phthalate levels in pregnant women. J Expo Sci Environ Epidemiol. 2012;22:468–75.
Braun JM, Just AC, Williams PL, Smith KW, Calafat AM, Hauser R. Personal care product use and urinary phthalate metabolite and paraben concentrations during pregnancy among women from a fertility clinic. Journal Expo Sci Environ Epidemiol. 2014;24:459–66.
Duty SM, Ackerman RM, Calafat AM, Hauser R. Personal care product use predicts urinary concentrations of some phthalate monoesters. Environ Health Perspect. 2005;113:1530–5.
Romero-Franco M, Hernandez-Ramirez RU, Calafat AM, Cebrian ME, Needham LL, Teitelbaum S, et al. Personal care product use and urinary levels of phthalate metabolites in Mexican women. Environ Int. 2011;37:867–71.
Lewis RC, Meeker JD, Peterson KE, Lee JM, Pace GG, Cantoral A, et al. Predictors of urinary bisphenol A and phthalate metabolite concentrations in Mexican children. Chemosphere. 2013;93:2390–8.
Sathyanarayana S, Karr CJ, Lozano P, Brown E, Calafat AM, Liu F, et al. Baby care products: possible sources of infant phthalate exposure. Pediatrics. 2008;121:e260–8.
Cosmetics CfS. Market Shift: the story of the compact for safe cosmetics and the growth in demand for safe cosmetics. 2011.
Harley KG, Kogut K, Madrigal DS, Cardenas M, Vera IA, Meza-Alfaro G, et al. Reducing Phthalate, paraben, and phenol exposure from personal care products in adolescent girls: findings from the HERMOSA Intervention Study. Environ Health Perspect. 2016.
Madrigal DS, Minkler M, Parra KL, Mundo C, Gonzalez JE, Jimenez R, et al. Improving Latino Youths’ environmental health literacy and leadership skills through participatory research on chemical exposures in cosmetics: the HERMOSA Study. International quarterly of community health education. 2016.
Kato K, Silva MJ, Needham LL, Calafat AM. Determination of 16 phthalate metabolites in urine using automated sample preparation and on-line preconcentration/high-performance liquid chromatography/tandem mass spectrometry. Anal Chem. 2005;77:2985–91.
Gavin QW, Ramage RT, Waldman JM, She J. Development of HPLC-MS/MS method for the simultaneous determination of environmental phenols in human urine. Int J Environ Anal Chem. 2014;94:168–82.
Hornung RW, Reed LD. Estimation of average concentration in the presence of nondetectable values. Appl Occup Environ Hyg. 1990;5:46–51.
Molitor J, Papathomas M, Jerrett M, Richardson S. Bayesian profile regression with an application to the National Survey of Children’s Health. Biostatistics. 2010;11:484–98.
Coker E, Liverani S, Ghosh JK, Jerrett M, Beckerman B, Li A, et al. Multi-pollutant exposure profiles associated with term low birth weight in Los Angeles County. Environ Int. 2016;91:1–13.
Locke B, Jachowicz J. Fading of artificial hair colour and its prevention by photofilters. Int J Cosmet Sci. 2006;28:231–2.
Bernhardt P, Giesen M, Hollenberg D, Hubbuch M, Kalhöfer V, Maier H, et al. UV filters for hair protection. Int J Cosmet Sci. 1993;15:181–99.
Meeker JD, Cantonwine DE, Rivera-Gonzalez LO, Ferguson KK, Mukherjee B, Calafat AM, et al. Distribution, variability, and predictors of urinary concentrations of phenols and parabens among pregnant women in Puerto Rico. Environ Sci Technol.2013;47:3439–47.
Just AC, Adibi JJ, Rundle AG, Calafat AM, Camann DE, Hauser R, et al. Urinary and air phthalate concentrations and self-reported use of personal care products among minority pregnant women in New York city. J Expo Sci Environ Epidemiol. 2010;20:625–33.
Kessler R. More than cosmetic changes: taking stock of personal care product safety. Environ Health Perspect. 2015;123:A120.
Kwapniewski R, Kozaczka S, Hauser R, Silva MJ, Calafat AM, Duty SM. Occupational exposure to dibutyl phthalate among manicurists. J Occup Environ Med. 2008;50:705–11.
Liao C, Kannan K. A survey of alkylphenols, bisphenols, and triclosan in personal care products from China and the United States. Arch Environ Contam Toxicol. 2014;67:50–9.
Dodson RE, Nishioka M, Standley LJ, Perovich LJ, Brody JG, Rudel RA. Endocrine disruptors and asthma-associated chemicals in consumer products. Environ Health Perspect. 2012;120:935.
Schettler T. Human exposure to phthalates via consumer products. Int J Androl. 2006;29:134–9.
Aurela B, Kulmala H, Soderhjelm L. Phthalates in paper and board packaging and their migration into Tenax and sugar. Food Addit Contam. 1999;16:571–7.
Colacino JA, Harris TR, Schecter A. Dietary intake is associated with phthalate body burden in a nationally representative sample. Environ Health Perspect. 2010;118:998–1003.
Kelley KE, Calafat AM, Mitchell AA, Hauser RB, Hernandez-Diaz S. Medications as a potential source of exposure to phthalates in the US population. 2008.
Biedermann-Brem S, Biedermann M, Pfenninger S, Bauer M, Altkofer W, Rieger K, et al. Plasticizers in PVC toys and childcare products: What succeeds the phthalates? Market survey 2007. Chromatographia. 2008;68:227–34.
Liao C, Kannan K. Concentrations and composition profiles of parabens in currency bills and paper products including sanitary wipes. Sci Total Environ. 2014;475:8–15.
Soni MG, Carabin IG, Burdock GA. Safety assessment of esters of p-hydroxybenzoic acid (parabens). Food Chem Toxicol: Int Journal Publ Br Ind Biol Res Assoc. 2005;43:985–1015.
CDC. Fourth Report on Human Exposure to Environmental Chemicals. Atlanta, GA: U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. 2009.
Gonzalez H, Farbrot A, Larko O, Wennberg AM. Percutaneous absorption of the sunscreen benzophenone-3 after repeated whole-body applications, with and without ultraviolet irradiation. Br J Dermatol. 2006;154:337–40.
Janjua NR, Frederiksen H, Skakkebæk NE, Wulf HC, Andersson AM. Urinary excretion of phthalates and paraben after repeated whole‐body topical application in humans. Int J Androl. 2008;31:118–30.
Sandborgh-Englund G, Adolfsson-Erici M, Odham G, Ekstrand J. Pharmacokinetics of triclosan following oral ingestion in humans. J Toxicol Environ Health A. 2006;69:1861–73.
This paper was supported by California Breast Cancer Research Program grant 18BB-1800 and NIEHS grant 1R21ES024909.
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The authors declare that they have no conflicts of interest.
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Berger, K.P., Kogut, K.R., Bradman, A. et al. Personal care product use as a predictor of urinary concentrations of certain phthalates, parabens, and phenols in the HERMOSA study. J Expo Sci Environ Epidemiol 29, 21–32 (2019). https://doi.org/10.1038/s41370-017-0003-z
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