Fig. 2: Visualization of the substeps of neutrophil extravasation by two-photon microscopy. | Experimental & Molecular Medicine

Fig. 2: Visualization of the substeps of neutrophil extravasation by two-photon microscopy.

From: LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) distinctly regulate neutrophil extravasation through hotspots I and II

Fig. 2

Analysis of neutrophil infiltration visualized from three-dimensional two-photon images of anti-CD31 antibody-labeled blood vessels in mouse cremaster upon stimulation with N-formylmethionyl-leucyl-phenylalanine: (1) intrusion into the endothelium, in which the neutrophil cell body started to penetrate the endothelial cell layer; (2) most of the neutrophil cell body then was colocalized within the CD31-labeled endothelial cell layer; (3) as the final step, the tailing edge of the extravasating neutrophil was attached. Please see Video 3