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Solid-state nanopores towards single-molecule DNA sequencing

Journal of Human Genetics volume 65, pages 69–77 (2020)Cite this article

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Abstract

Nanopore DNA sequencing offers a new paradigm owing to its extensive potential for long-read, high-throughput detection of nucleotide modification and direct RNA sequencing. Given the remarkable advances in protein nanopore sequencing technology, there is still a strong enthusiasm in exploring alternative nanopore-sequencing techniques, particularly those based on a solid-state nanopore using a semiconductor material. Since solid-state nanopores provide superior material robustness and large-scale integrability with on-chip electronics, they have the potential to surpass the limitations of their biological counterparts. However, there are key technical challenges to be addressed: the creation of an ultrasmall nanopore, fabrication of an ultrathin membrane, control of the ultrafast DNA speed and detection of four nucleotides. Extensive research efforts have been devoted to resolving these issues over the past two decades. In this review, we briefly introduce recent updates regarding solid-state nanopore technologies towards DNA sequencing. It can be envisioned that emerging technologies will offer a brand new future in DNA-sequencing technology.

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Acknowledgements

The authors would like to express the utmost thanks to all coworkers for their dedication to Hitachi’s solid-state nanopore DNA sequencer project.

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  1. Center for Technology Innovation - Healthcare, Research & Development Group, Hitachi Ltd., 1-280 Higashi-Koigakubo, Kokubunji, Tokyo, 185-8601, Japan

    Yusuke Goto, Rena Akahori, Itaru Yanagi & Ken-ichi Takeda

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  1. Yusuke Goto
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Correspondence to Yusuke Goto.

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Financial support for this work was provided by Hitachi, Ltd. Y.G., R.A., I.Y., and K.T. are current employees of Hitachi, Ltd.

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Goto, Y., Akahori, R., Yanagi, I. et al. Solid-state nanopores towards single-molecule DNA sequencing. J Hum Genet 65, 69–77 (2020). https://doi.org/10.1038/s10038-019-0655-8

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