Abstract
Background: BPD is a chronic lung disease affecting preterm infants leading to increased morbidity and mortality. Main risk factors include premature birth, mechanical ventilation (MV), hyperoxia and inflammation. A newborn rat ventilation model with reversible intubation was used to study delayed gene expression modifications to increase our understanding of its pathophysiology.
Methods: LPS was injected intraperitoneally (2mg/kg) to male rat pups on postnatal day 4 or 5 to mimic systemic inflammation. Twenty-four hours after injection they were intubated and ventilated for 6h with tidal volume of 15ml/kg and 21% or 60%O2. After weaning from anesthesia, they were returned to their mothers for 48h. Gene expression was measured by Affymetrix®Gene-Arrays in four groups (n=9/Group) and verified by qPCR.
Results: Expression changes were mainly found in genes involved in inflammation and extracellular matrix remodeling. Among them MMP-9 and several of its regulator genes were significantly modified.
Conclusion: MMP-9 is known to be important in lung development, angiogenesis and tissue repair. In our BPD-model, MMP-9 pathway seemed to be a central target. Several genes increasing MMP-9 activity were synergistically upregulated, highlighting its potential role in lung injury and repair. Therefore, the role of MMP-9 in the pathophysiology of BPD deserves further investigation.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Denervaud, V., Gremlich, S., Schittny, J. et al. Analysis of Potential Biomarkers of Bronchopulmonary Dysplasia (BPD) in a Newborn Rat Ventilation Model. Pediatr Res 70 (Suppl 5), 554 (2011). https://doi.org/10.1038/pr.2011.779
Issue Date:
DOI: https://doi.org/10.1038/pr.2011.779