Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Poster Presentations: Infectious Diseases, Immunology

Fecal Calprotectin Determination in Preterm Neonates: Evaluation of Two Methods


Background and aims: Fecal calprotectin (FC) is a recognized marker of intestinal inflammation, previously validated in different clinical settings. Recent studies have suggested its uselfulness in early detection of neonatal necrotizing enterocolitis. Although standard methodology for FC determination is ELISA, quicker alternative techniques have been recently proposed, but not yet evaluated at this age group. A preliminary comparative analysis of FC concentration using ELISA and QuantumBlue* in preterm neonates was thus performed.

Methods: Fecal samples were prospectivelly collected postnatally at: 48-96hours (T1), end of 1st (T2) and 4th week of life (or at discharge) (T3), frozen at -20°C and subsequently processed for determination of FC concentration using simultaneously Quick test (QuantumBlue®) and ELISA (Buhlmann*)(two determinations using the two methods for each sample). Clinical data were also obtained. Descritive statistical analysis and Spearman correlation coefficient were performed.

Results: 14 neonates had a median gestacional age 32 weeks (25-34), birth weight:1636,7±428,6 grs; 2 cases with GI disease; number of samples assessed: 1 / 2, 2 / 5, 3/ 8 neonates. The mean FC concentration (mcg/g) at T1 (n=24) was, respectively :257,93±191,87(quick test); 285,21±227,61(ELISA); at T2: (n=12): 314,33±219,90 (quick test); 357,33±218,78(ELISA); at T3 (n=8): 359,43±121,66 (quick test); 349,29±191,87(ELISA). An overall 97.8% correlation (p:0.001) between the two tests was found.

Conclusions: FC values found in this preliminary cohort of preterm neonates have been similar to those reported in the literature. The finding of a good correlation between the two techniques suggests the potential clinical usefulness of QuantumBlue at this age group (after validation).

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

Gonçalves, C., Oliveira, G., Cardoso, B. et al. Fecal Calprotectin Determination in Preterm Neonates: Evaluation of Two Methods. Pediatr Res 70, 475 (2011).

Download citation


Quick links