Abstract
Background: We wanted to explore if longer initial ventilation intervals than 30 s prior to initiation of cardiac compressions during resuscitation of newborn pigs would modulate levels of inflammatory markers in the newborn pig brain.
Material and methods: Newborn pigs were anaesthetized and mechanically ventilated. Progressive asphyxia was induced until asystole occurred. Animals received ventilation for 30 s, 60 s or 90 s before onset of chest compressions. After return of spontaneous circulation, the pigs were observed for 4 hours before cerebrospinal fluid (CSF) and samples from the brain were collected.
Results: CSF: IL-6 (pg/ml) and TNFα (pg/ml) were significantly lower in the 60 s group (30.2 ± 25.6 and 34.6 ± 27.2) compared to the 30s group (154 ± 168.3 and 86.2 ± 50.7), p=0.02 and p=0.01, respectively. There were significantly lower levels of S100 in the 30 s group (19.1 ug/L ± 20.3) compared to the 90 s group (42.1 ug/L ± 30.7), p=0.01.
Frontal cortex: We found no significant differences in inflammatory markers between the groups in the frontal cortex.
Hippocampus: Gene expression of MMP-2 was significantly lower in the group ventilated for 60 s compared to the 30 s group, p=0.02.
Conclusion: We found a higher inflammatory response in pigs resuscitated from cardiac arrest to ROSC when providing ventilation for 30 s and 90 s compared to 60 s of initial ventilation, indicating that a slightly longer ventilation period than the recommended guidelines of 30 s might be beneficial.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dannevig, I., Solevåg, A., Sonerud, T. et al. Newborn Resuscitation - Longer Periods of Initial Ventilation and the Impact on Markers of Brain Inflammation in Newborn Pigs. Pediatr Res 70 (Suppl 5), 163 (2011). https://doi.org/10.1038/pr.2011.388
Issue Date:
DOI: https://doi.org/10.1038/pr.2011.388