Abstract
Background and aims: Perinatal asphyxia is associated with hypoxia-reoxygenation injury. Supplementary oxygen use influences both morbidity and mortality.
To study whole genome expression alterations induced by supplementary oxygen in vulnerable tissues in a newborn mouse model of hypoxia and reoxygenation.
Methods: C57BL/6 mice postnatal day 7 were randomized to hypoxia (8%O2, 36±0.5°C) for 120 min and reoxygenation in 21% (H21, n=6), 40% (H40, n=6), 60 % (H60, n=7) or 100% (H100, n=6) O2 for 30 min. Mice were then sacrificed and rapidly dissected on ice after 150 min recovery. Two control groups not exposed to hypoxia, but either 21% (C21, n=5) or 100% O2 (C100, n=8) for 30 min were used as comparison. GeneChip Mouse Gene ST 1.0 Arrays (Affymetrix) were used to analyze whole genome expression. Statistical analysis was performed by using BAMarray software. The Gene Ontology and DAVID Bioinformatic Resources 2008 databases were used for functional analysis.
Results: 560 genes were significantly differentially expressed in lung tissue between group C21 and either of the four intervention groups (H21: 39, H40: 148, H60: 343, H100: 145). Preliminary gene ontology analyses of the genes in the H40 and H60 group indicate that cell growth and differentiation, energy metabolism and inflammation are processes represented.
Conclusion: A significant difference in gene expression in 560 genes in lung tissue of newborn mouse was found in our hypoxia and reoxygenation model using 21, 40, 60 and 100% oxygen. The impact of the gene expression alterations is undergoing further analysis.
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Wollen, E., Sejersted, Y., Wright, M. et al. 349 Hypoxia and Reoxygenation Induce Alterations in Whole Genome Expression in Lung Tissue of the Newborn Mouse. Pediatr Res 68 (Suppl 1), 180 (2010). https://doi.org/10.1203/00006450-201011001-00349
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DOI: https://doi.org/10.1203/00006450-201011001-00349