Abstract
Background and aims: Study of lung function in anexperimental model of bronchopulmonary dysplasia(BPD) induced by hyperoxia in newborn rats.
Methods: The subjects of this study were twolitters of Sprague-Dawley rats. Group C (Control):exposure to room air. Group H (Hyperoxia): exposureto 60% of Oxygen from birth and during 14 days. The lung function tests were performed on day 15. Respiratory functional evaluation through a systemof total body plethysmography (Buxco Electronics, Troy, NY, USA): non-invasive analysis system, withthe animal conscious and free, housed in a sealedcontainer. Changes of pressure and volume in thecabin due to respiratory movements are monitoredand used to calculate ventilatory parameters usinga computer application.
Results: Two litters with 14 offspring each werestudied. Pulmonary function tests showed adecrease in tidal volume (H: 0.098 ml, C: 0.125 ml, p0,000) with offsetting increase in respiratory rate (H:310 rpm; C: 266 rpm; p 0,03) without differences inminute volume (H: 29.92 ml / min, C: 32.75 ml / min;p 0,186). The index of airway obstruction (Penh) isaltered in the litter exposed to oxygen (p 0, 026).
Conclusions:
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- Validation of an experimental model of hyperoxia(DBP) in newborn rats.
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- Functional respiratory non-invasive test whichmainly showed a decrease in tidal volume withincreased respiratory rate volume.
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- Non invasive total body plethysmography is anadequate method to study lung function in thisanimal model.
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Camacho, C., Rodríguez, M., López, S. et al. 1315 Lung Function in Hyperoxia Experimental Model of Newborn Rats. Pediatr Res 68 (Suppl 1), 650–651 (2010). https://doi.org/10.1203/00006450-201011001-01315
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DOI: https://doi.org/10.1203/00006450-201011001-01315