Figure 1 | Pediatric Research

Figure 1

From: Cytomegalovirus (CMV) Inactivation in Breast Milk: Reassessment of Pasteurization and Freeze-Thawing

Figure 1

Virological parameters of different procedures for CMV inactivation in breast milk. (A) Stability of viral DNA after freezing or heating. (B) Unimodal kinetics of CMV viral load during lactation of a maternal transmitter (see Table 1, mother C) of a term infant. The arrows indicate d 59, 66, and 87 p.p.; d 61: onset of viruria of the infant. (C) Virus load (infectivity) expressed as a percentage of the control for the five inactivation methods (Kruskal-Wallis test, p = 0.002). Number of experiments using spiked milk (AD) / naturally infected milk (wt): 18 h, −20°C, 2 AD; 4 d, −20°C, 2 AD / 3 wt; 10 d, −20°C, 3 AD; 30 min, 62.5°C, 3 AD / 1 wt; 5 s, 72°C, 4 AD / 4 wt. (D) Virus elimination by heating vs cryoinactivation (observed percentages together with exact 95% confidence intervals; p < 0.0001).

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