Abstract
Aim:We have been investigating pathology about experimental systemic vasculitis. The experimental arteritis mimicking that of Kawasaki disease (KD) can be induced by intraperitoneal injection of alkaline extract which is prepared from cell wall of Candida albicans isolated from feces of patients with KD. The present study aims to elucidate particular component of alkaline extract which induces arteritis. Methods:Alkaline extract (CADS) was prepared by the method which we had already reported. The component of CADS was analyzed by 1H-NMR. To remove the protein from CADS, Fehling's solution was added to its suspension (Fehling fraction). To remove β1,3-glucan from CADS, it was digested with zymolyase. (Zymolyase fraction). Fractions of protein-rich and mannan-rich were separated from CADS by ammonium sulfate precipitation. Fractions of β1,6-glucan-rich and β 1,3-glucan-rich were obtained by the sodium hypochlorite treatment from the residue of alkaline extracted C. albicans. CADS and other fractions were suspended in PBS and injected to mice (C3H/HeN, 4 weeks of age, male) intraperitoneally for five consecutive days in the first week and the fifth week (4mg/mouse/day). Mice were sacrificed in the 9th week. Microscopic observation was performed by routine histological technique. Results:By 1H-NMR analysis, CADS is suggested to contain a large amount of mannan, and a small amount of β1,6-glucan, protein, and β1,3-glucan. Incidence of coronary arteritis induced by CADS, other fractions of Fehling, Zymolyase, protein-rich, mannan-rich, β1,6-glucan-rich, and β1,3-glucan-rich is 5/10, 1/10, 4/10, 2/10, 4/10, 3/10, and 1/10, respectively. There was no histological difference among each group. Conclusion:These results suggest that mannan and β 1,6-glucan have influence on development of coronary arteritis in this model.
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Oharaseki, T., Takahashi, K., Miura, N. et al. Analysis of Component of Candida albicans Cell Wall Inducing Systemic Vasculitis in Mice. Pediatr Res 53, 175 (2003). https://doi.org/10.1203/00006450-200301000-00130
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DOI: https://doi.org/10.1203/00006450-200301000-00130