Abstract 210

Packed RBC from placental blood were stored as CPDA-1 preparations (n=23) or after suspension in the additive solutions SAG-M (n=20) or PAGGS-M (n=16). Biochemical and rheological parameters were studied at days 0, 14 and 28 after collection. Viscosities were determined using a capillary viscosimeter with tube diameters of 270 and 87 µm at adjusted hematocrits of 0.40 L/L. 2,3DPG and ATP concentrations of RBC were determined enzymatically. Significant differences were found among the three preparations.

Viscosities increased significantly in CPDA-1 preparations, but remained unchanged in SAG-M and PAGGS-M preparations over the entire storage period. 2,3DPG concentrations (mean values at day 28 in % of initial) decreased significantly in CPDA-1 (8.4%), SAG-M (15.4%) and PAGGS-M (19.5%) within 28 days. Red cell ATP concentrations (mean values at day 28 in % of initial) decreased significantly in CPDA-1 (42%), SAG-M (61%) and PAGGS-M (78%) within 28 days.

From our findings we come to three major conclusions: Placental blood can be processed in a similar way as blood from donors. Additive solutions improve the quality of preparations. PAGGS-M seems to be superior in the preservation of flow properties, oxygen transport capacity (2,3 DPG) and viability (ATP) of packed fetal red blood cells.