European Society for Pediatric Research Abstracts

Differential Effects of Nitric Oxide Species on Pc12 Cell Survival

Background: Nitric oxide (NO) is known to be both neurotoxic and neuroprotective. There are three redox-related species: neutral NO, the nitroxyl anion (NO-) and the nitrosonium cation(NO+).

Subjects and Interventions: Differentiated PC12 cells were treated with increasing concentrations (0.001 to 5 mM) of NO, NO+, and NO-, in the presence or absence of nerve growth factor(NGF).

Results: Low concentrations (up to 100 μM) of NO and NO+ inhibited apoptosis after NGF withdrwal, while NO- did not. In the presence of NGF, higher concentrations (above 300 μM) of NO and NO+ induced apoptosis while NO- treatment resulted in necrosis.

Conclusion: Nitric oxide can be neuroprotective or neurotoxic, depending on the redox state of the molecule. NO and NO+ induce apoptosis, while NO- induces necrosis in PC12 cells.

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