Figure 4 | Pediatric Research

Figure 4

From: Methionine and Serine Formation in Control and Mutant Human Cultured Fibroblasts: Evidence for Methyl Trapping and Characterization of Remethylation Defects

Figure 4

Formation of methionine and serine in cells grown with varying amounts of cbl in the medium. Cells were grown for 4 d in normal medium then for 3 d in medium supplemented with hydroxo-cbl at the depicted concentrations. Cells were incubated with labeled formate and harvested, and proteins were precipitated, oxidized, hydrolyzed, and analyzed by high voltage electrophoresis as described in “Methods.”

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