Excess airway smooth muscle proliferation may play a role in the pathogenesis of airways obstruction in chronic, severe asthma and bronchopulmonary dysplasia. Lysophosphatidic acid (LPA) is a bioactive phospholipid thought to be responsible for a large fraction of the growth-promoting activity of serum. We examined the effects of LPA on the DNA synthesis of cultured bovine tracheal myocytes by measurement of[3H]-thymidine incorporation. LPA increased DNA synthesis in a concentration-dependent manner, but had substantial effects only at supraphysiologic concentrations (Figure). In addition, LPA also had a synergistic effect on the DNA synthesis of cells concurrently treated with either platelet-derived growth factor (PDGF) or epidermal growth factor (EGF). These data suggest that LPA, in combination with polypeptide growth factors, may contribute to the airway smooth muscle layer thickening observed in chronic airways diseases.
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Ramakrishnan, M., Liu, P., Li, J. et al. LYSOPHOSPHATIDIC ACID POTENTIATES POLYPEPTIDE GROWTH FACTOR-INDUCED AIRWAY SMOOTH MUSCLE DNA SYNTHESIS 1821. Pediatr Res 41, 306 (1997) doi:10.1203/00006450-199704001-01840