F2-ISOPROSTANES (F2I) IN TRACHEOBRONCHIAL ASPIRATE FLUID (TBAF) INDICATE ASSOCIATION BETWEEN INCREASED FIO2 AND LIPID PEROXIDATION IN THE LUNGS OF PREMATURE INFANTS. 1957

In vivo evidence that increased FIO2 contributes to oxidant-induced lung injury in premature infants is limited. F2I, which are free-radical catalyzed metabolites of arachidonic acid produced independent of cyclooxygenase, have been shown to be a highly sensitive and specific marker of lipid peroxidation in vivo. To determine whether increased FIO2 is associated with pulmonary lipid peroxidation, a mass spectrometric assay was employed to measure F2I in TBAF from 18 NICU patients (BW=1014±369 g) at a postnatal age (PNA) between 24 and 180 h. 3 patients had received only room air (RA). Of the 15 patients receiving increased FIO2, TBAF was obtained ≤ 50 h PNA in 4 (EarlyO2) and >50 h PNA in 11 (LateO2). FIO2 was not significantly different between EarlyO2 and LateO2 (0.49 vs. 0.47). F2I levels were significantly different between the 3 groups (P=0.005). LateO2 had the highest F2I levels (96, {18-405}pg/mL, median, {range}). Low F2I levels were found in RA and EarlyO2 and were not significantly different (20, {0-30} vs. 5, {0-29}, respectively). When EarlyO2 was excluded, there was a significant correlation between F2I levels and FIO2 (r=0.645, P=0.013). These results provide the first direct evidence of an association between FIO2 and lipid peroxidation in the lungs of premature infants, and demonstrate the utility of F2I as a marker of lipid peroxidation in infants at risk for hyperoxic lung injury. The delay in onset of increased F2I levels between EarlyO2 and LateO2 is consistent with a loss of antioxidant factors and/or an O2 induction of both enzymatic and non-enzymatic sources of free radicals such as that shown to occur with lung cytochrome P450 (JCI 96:2083, 1995). (Supported by NIH Pediatric Pulmonary SCOR HL-14214)

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Cotton, R., Morrow, J., Hazinski, T. et al. F2-ISOPROSTANES (F2I) IN TRACHEOBRONCHIAL ASPIRATE FLUID (TBAF) INDICATE ASSOCIATION BETWEEN INCREASED FIO2 AND LIPID PEROXIDATION IN THE LUNGS OF PREMATURE INFANTS. 1957. Pediatr Res 39, 329 (1996). https://doi.org/10.1203/00006450-199604001-01981

Download citation

Further reading