Abstract
Recent development of a specific radioimmunoassay for IGFBP-3 permits its measurement in disorders of the GH-IGF-1 axis. To establish normal IGF-1 and IGFBP-3 values in our population, serum was drawn from 86 normal children of both sexes ana different stages of puberty and compared to 7 patients with short stature with normal GH tests, 10 with GH deficiency (confirmed by 2 stimulation tests) and 3 with precocious puberty. IGFBP-3 assay was performed by CRIESP - Central ae Radioimmunoassay de Sao Paulo witn a commercial kit from DSL according to the method of Miles et al. There is no cross-reactivity with other binding oroteins (IGFBP-1, 2 and 4). The sensitivity was 0.5 mg/L and intra - and interassay coefficients of variation were below 4.0%. IGF-1 was assayed at the Radioimmunoassay Laboratory of the Hospital das Clinicas after ethanol extraction using a kit from Nichols.
Results among normal children were compared by unpaired Student t-test. There was no statistical difference between IGFBP-3 and DHEA-S in males and females, whereas IGF-1 levels in Tanner pubertal stages I to III were significantly higher in females. IGFBP-3 was normal in the 7 patients with short stature, low in 60% of patients with GH deficiency, 25% of which had low IGF-1, compared to controls. The 3 patients with precocious puberty had normal IGFBP-3 levels for pubertal stage.
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Silva, E., Pelegrinelli, A., Borges, M. et al. IGF-l AND IGFBP-3 LEVELS IN NORMAL CHILDREN AND CHILDREN WITH DISORDERS OF GROWTH. Pediatr Res 38, 624 (1995). https://doi.org/10.1203/00006450-199510000-00041
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DOI: https://doi.org/10.1203/00006450-199510000-00041