Abstract
FNABT was done in patients with ATD (Chronic Lymphocytic Thyroiditis= CLT and Graves'Disease= GD), and processed according to the mothod of Hayry at al., applied to follow up transplanted organs. Citospin samples were stained with May Grunewald-Giemsa, and HLA-DR, CD25, CD4, CD8, CD3, CD11 and surface immunoglobulines were detected by immunofluorescence. In transplanted kidneys, a corrected increment (inflamatory INDEX) (CI)3,5 indicates immunological activation. We analized thyroid samples, obtaining the CI, and the phenotype of infiltrating cells. 56 FNABT were perfomed on 43 pts. (CLT n=23 and GD n=20), followed up from 1 y. to 4,5y. They were divided in 2 gr., according to their thyroid status. Gr.I:CLT n=29 and Gr.II:GD n=27. Results: (%±SD). Gr.I: CI: 6.3±0.97; B-cells: 33.9±10.5; T-cellS: 61.2±11.4; CD4:63.1±6.2; CD 8:34.4±6.6. CD4/CD8 ratio: 1.9±0.5 HLA-DR expression was found on 56.6±18.8% of the follicular cells and CD25 was expressed in 27/29 pts. Gr.II:CI 4.2±1.3(Gr.I vs II = p<0.001) B-cells: 58.5±16.2 (p<0.001) T-cells: 39.6±17.1 (p<0.001)CD4: 59.2±8.8 (p<0.005)CD8: 40±9.4 (p<0.02) CD4/CD8 ratio: 1.6±0.7 (p<0.02). HLA-DR was expressed on 26.6±17.3 (p<0.001) of follicular cells and CD25 was positive in 7/16 pts. All CLT showed typical cytological aspect. In GD 7/20 cytology 12/20 were normal and 1/20 adenomatous goiter (AG). The CI5.3, the expression degree of HLA-DR on follicular cells, as well as the expression of CD 25 on lymphocytes are markers of immune activation. TLC could be considered as a form of rejection, in which cellular aggression would play an important role, being in GD mainly humoral.
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Goldberg, J., Herzovich, V., Rossi, J. et al. 16 JUVENILE AUTOIMMUNE THYROID DISEASE (ATD). FINE NEEDLE ASPIRATION BIOPSY (FNABT) AND TIMUNOAMARCATION (PART I). Pediatr Res 36, 674 (1994). https://doi.org/10.1203/00006450-199411000-00040
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DOI: https://doi.org/10.1203/00006450-199411000-00040