Abstract
Lymphocyte proliferation (LYP) after stimulation with mitogens (phytohaemagglutinin (PHA), mitogen (PWM), concanavalin A (CON A)) measured by 3H-thymidine incorporation was performed in whole blood (n=19) and preterm (n 13) neonates using (100 μl) of cord blood. LYP increased 150 100 fold above baseline. Dexamethasone (DEX) (1010 - 10−1 mol/l) suppressed stimulated LYP in a concentration dependent manner. In premature neonates treated with DEX bronchopulmonary dysplania serum levels of 0.2 0.8 × 10.8 mol/l were measured. At this concentration mediums of only 15% (CON A), 51% (PHN) and 68% (PWM) of the uninhibited LYP were obtained. DEX, hydrocortisone, and fludrocortisone in equipetent glucocorticoid contrations supppressed LYP to the same extent. We conclude that DEX suppresses stimulated LYP in neonates considerably at concentrations applied during treatment for BPD which moswell compromise immune function especially infection control.
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Schwarze, J., Bartmann, P. THE INFLUENCE OF DEXAMETHASONE ON LYMPHOCYTE PROLIFERATION IN WHOLE BLOOD CULTURES OF NEONATES. Pediatr Res 35, 277 (1994). https://doi.org/10.1203/00006450-199402000-00135
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DOI: https://doi.org/10.1203/00006450-199402000-00135