Abstract
ABSTRACT: Colonic mucins may serve as a defense mechanism by binding bacterial, viral, or dietary lectins, thereby preventing them from attaching to the intestinal epithelium. Presumably, the composition of the mucins would be responsible for this phenomenon, and the composition of mucins from mature mammals would be the most effective in binding lectins. To determine whether differences in diet and/or age affect the composition of colonic mucins, we scraped fresh colonic mucosae from pigs at 0 (n = 3), 7 (n = 3), 21 (n = 3), and 180 (n = 3) d of age and purified the mucins from these mucosal scrapings. Mucins were purified by ribonuclease and deoxyribonuclease digestion, high-performance size-exclusion chromatography, and cesium chloride density-gradient ultracentrifugation. The 180-d-old pig was considered mature. No changes were observed in any of the variables analyzed in the 7-d-old animals. No changes were observed in quantities of galactosamine and galactose. The amounts of fucose and glucosamine increased by 165 and 37%, respectively, (p < 0.05) from d 0 to d 21 in the sow-fed animals, at which time fucose and glucosamine content were 48 and 22% greater, respectively, than in the 21-d-old, artificially fed group (p < 0.05). A further significant increase in fucose content was observed in the mucins from mature animals. The sulfate content in the 21-d-old, sow-fed animals was significantly lower than in both the newborn and the 21-d-old artificially fed animals. The sulfate content in all three of these groups, however, was significantly higher than that observed in the mucins of mature animals. At 21 d of age, the protein content was significantly lower than at birth for both diet groups but significantly higher than in the mucins of mature pigs. The evidence indicates that the composition of colonic mucins in 21-d-old sow-fed pigs was more mature than that in the artificially fed counterparts, with respect to fucose, glucosamine, and sulfate. Therefore, if a mature mucin composition is, in fact, a more effective defense against intestinal infection, young breast-fed animals may have an advantage over young artificially fed animals.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Turck, D., Feste, A. & Lifschitz, C. Age and Diet Affect the Composition of Porcine Colonic Mucins. Pediatr Res 33, 564–567 (1993). https://doi.org/10.1203/00006450-199306000-00005
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1203/00006450-199306000-00005
This article is cited by
-
Black soldier fly and gut health in broiler chickens: insights into the relationship between cecal microbiota and intestinal mucin composition
Journal of Animal Science and Biotechnology (2020)
-
Effects of dietary Hermetia illucens meal inclusion on cecal microbiota and small intestinal mucin dynamics and infiltration with immune cells of weaned piglets
Journal of Animal Science and Biotechnology (2020)
-
New insights on the colonization of the human gut by health-promoting bacteria
Applied Microbiology and Biotechnology (2020)
-
Longitudinal investigation of the swine gut microbiome from birth to market reveals stage and growth performance associated bacteria
Microbiome (2019)
-
Impact of activated charcoal and tannin amendments on microbial biomass and residues in an irrigated sandy soil under arid subtropical conditions
Biology and Fertility of Soils (2014)