Abstract
Using a sensitive DELPHIA (time-resolved fluoroimmunoassay) for measurement of LH, we have reanalyzed 250 spontaneous secretory studies. Samples were obtained every 20 minutes and aliquots from timed intervals were pooled: 24HR = 0800-0800h, AH = 0800-1600h, PM = 2200-0600h. Pools were assayed for LH (sensitivity 0.0365 IU/L); and FSH, GH, testosterone (T), and estradiol (E2) were measured by radioimmunoassay. In 89 control males, there was a rise in 24HR LH at pubertal stage (PS) II (3.47±3.97) over PS I (0.5 5 ± 0.69, p<0.001), with a decrease again in PS III (1.46 ± 0.38). This pattern was not seen in the 51 control females. In the 85 pubertal controls, there was a diurnal variation with PH LH (3.92 ± 3.78) higher than AM LH (1.79 ± 1.65, p<0.01). There was a weak correlation of 24HR LH with 24HR GH (r=0.24, p<0.02) and stronger correlations with 24HR FSH {r=0,56), 24HR E2 (r=0.37), and 24HR T (r=0.35). Interestingly, cranially irradiated PS I children (n = 12) had higher 24HR LH (3.32±6.00) compared to 66 PS I controls (0.62 ± 0.85, p<0.001) and FSH (13.25±18.02 vs 2.03±1.78, p<0.001), whereas their mean T and E2 where not different. Pooled LH values are useful additions to spontanoue secretion studies and give information on irradiation.
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Jorgeneen, E., Shulman, D., Diamond, F. et al. POOLED 24 HOUR AND DIURNAL LH VALUES: STUDIES IN CONTROL AND IRRADIATED CHILDREN. Pediatr Res 33 (Suppl 5), S48 (1993). https://doi.org/10.1203/00006450-199305001-00268
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DOI: https://doi.org/10.1203/00006450-199305001-00268