Abstract
Estradiol effects on DNA-3H-thymidine incorporation by cultured human fetal epiphyseal chondrocytes incubated 96h In a serum-free medium were evaluated. Cells were in the presence of E2 (0.001-100 nM) for the last 48h of Incubation and 3H-thymidine (5 μCI/ml) was added for the last 24h. E2 (0.1 and 0.01 nM) significantly Inhibits DNA-3H-thymidine Incorporation in cultured chondrocytes from 25-26-weeks-old male and female fetuses. In males (n=8) E2 inhibition at 0.01 nM was 62.3 ± 21.4 (M ± SD), (range 23.2-84.5), controls=100. (p= 0.0001-0.03). In females (n = 5) E2 Inhibition at 0.01 nM was 55.6 ± 7.8, (range 49.8-66.8), controls = 100, (p= 0.001-0.01). No stastically significant differences in E2 effects on cells from male and female fetuses were observed. In conclusion. E2 exerts a biological effect on cultured human fetal epiphyseal chondrocytes. These results suggest that estrogens might be involved in growth regulation of human fetal epiphyseal cartilage
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Audi, L., Carrascosa, A. & Ballabriga, A. ESTRADIOL INHIBITION OF DNA-3 H-THYMIDINE INCORPORATION IN CULTURED HUMAN FETAL EPIPHYSEAL CHONDROCYTES. Pediatr Res 33 (Suppl 5), S44 (1993). https://doi.org/10.1203/00006450-199305001-00246
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DOI: https://doi.org/10.1203/00006450-199305001-00246