Abstract
Simultaneous overnight 12 hour plasma growth hormone profiles and timed urine collections were obtained in 100 patients (mean age 11.0yrs, range 3.4-33.9yrs) undergoing investigation of growth hormone (GH) secretion. All have normal renal function. Plasma samples obtained at 20 minute intervals from 20.00-08.00hrs were assayed by an in-house IRMA employing an MAb as label (between-batch CV <8.5% from 2.2-49 mU/l). GH profiles obtained were analysed using the Munro modification of PULSAR. Urine GH was assayed using the Novo Nordisk Novoclone amplified enzyme immunoassay (between-batch CV <12% from 9-28ng/l).
Mean plasma GH concentration was 7.7 mU/l (SD 4.0, range 0.6-25.3). Mean plasma pulse amplitude was 15.6 mU/l (SD 8.6, range 1.9-48.0). Mean 12 hr urine GH concentration was 6.65 ng/l (SD 4.23, range 0.6-19.7). There were highly significant correlations (p=0.001) between urine GH concentration and mean pulse amplitude (r=0.54), mean overnight plasma GH levels (r= 0.53), sum of pulse amplitude (r=0.49) and sum of pulse area (r=0.49). Children with severe GH insufficiency were readily identified by low urine GH levels. This large series shows that timed overnight urine GH concentration reflects nocturnal plasma GH profiles.
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Stirling, H., Seth, J., Sturgeon, C. et al. THE RELATIONSHIP BETWEEN OVERNIGHT PLASMA GROWTH HORMONE PROFILES AND NOCTURNAL URINARY GROWTH HORMONE EXCRETION. Pediatr Res 33 (Suppl 5), S40 (1993). https://doi.org/10.1203/00006450-199305001-00223
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DOI: https://doi.org/10.1203/00006450-199305001-00223