Abstract
Cerebral complications like brain edema, bleeding, thrombosis, etc. are very frequent in the course of neonatal bacterial infections. We investigated in vivo the reactions of pial vessels by fluorescence macroscope giving LPS (E.coli O III B 4) to newborn piglets in intravenous infusion in doses 0.1 μg/kg bw./h (Group I, n=6) and 1.0 μg/kg bw./h (Group II. n=6) through 4 hours. 6 animals were given 0.9% NaCl, and served as controls (Group III). The physiological parameters (HR, MABP, CVP, body-temperature, blood gases and acid-base state) were monitorized continuously. Using 1% NaF as BBB permeability tracer extravasation was observed in Group I (128.3±27.7 min alter the start of infusion), and in Group II (177.3±28.3 min, N.S. vs. Group I). Brain NaF uptake was higher in Group I (2.1±0.4 μgNaFxmg−1 protein/μgNaFxμl−1 serum), than in Group II (1.4±0.5 μgNaFxmg−1 protein/μgNaFxμl−1 serum, N.S. vs. Group I) and was non-detectable in Group III. Continuous administration of both doses of LPS produced hyperdynamic state with increased cardiac output (180% of baseline) and significantly decreased lung-thorax compliance (80% of initial value) at the time of BBB-opening. During endotoxin infusion WBC-counts in sera of treated groups elevated gradually; they reached an approximately 2-fold increase when NaF extravasation occurred, and an almost 7-fold increase 2 hours after the end of the infusions. At this time a moderate metabolic acidosis and pleocytosis was found in Group I (1800±431 cells/μl CSF) and in Group II (345±79 cells/μl CSF, p<0.01). All the parameters studied were within normal range in Group III. Ii is concluded, that LPS given in similar doses as found frequently in plasma from septic newborns opens the BBB for NaF in piglets. These brain microcirculatory disturbances were accompanied with significant leucocytosis and pleocytosis augmented them. (All values are mean±SE.)
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Kovács, J., Ábrahám, C., Speer, C. et al. 110 E.COLI ENDOTOXIN (LPS) GIVEN IN INTRAVENOUS INFUSION RESULTS IN BLOOD-BRAIN BARRIER (BBB) OPENING FOR NAFLUORESCEIN (NaF) IN NEWBORN PIGLETS. Pediatr Res 30, 646 (1991). https://doi.org/10.1203/00006450-199112000-00140
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DOI: https://doi.org/10.1203/00006450-199112000-00140