Abstract
Curosurf, a surfactant preparation derived from minced pig lungs, used for the treatment of newborn infants with RDS, contains low molecular weight proteins. We studied the immune response after a single dose of Curosurf given intratracheally in 68 patients with RDS, 49 treated with Curosurf and 19 controls. Sera were taken before, 3 wks and 3 months after surfactant treatment. The sera were applied on frozen pig lung tissue, followed by an indirect peroxidase assay. Using this procedure any immune response to lung tissue components would be demonstrated as a brown-reddish deposit. In none of the controls nor in the sera obtained before Curosurf treatment we found any reaction. In 4 of the 49 Curosurf-treated patients we found a positive immune response, demonstrated by a clear brown staining of bronchial walls and brown granular deposits in alveolar cells, and in some lung tissue specimens collagen strands were also slightly brown coloured. Of these 4 patients, 2 showed a positive reaction 3 wks and 3 months after treatment, 1 only 3 wks after and 1 only 3 months after Curosurf treatment. We conclude that sera of some patients treated with Curosurf appear to contain antibodies against pig lung tissue components and surfactant proteins.
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Bos, A., Oetomo, S., De Ley, L. et al. 100 IMMUNE RESPONSE AFTER HETEROLOGOUS SURFACTANT TREATMENT OF NEWBORNS WITH RESPIRATORY DISTRESS SYNDROME (RDS). Pediatr Res 28, 293 (1990). https://doi.org/10.1203/00006450-199009000-00124
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DOI: https://doi.org/10.1203/00006450-199009000-00124