Abstract
ABSTRACT: Lactoferrin has been identified as a factor in human colostrum that accounts for increased incorporation of thymidine into the DNA in an in vitro rat crypt enterocyte bioassay. We have examined lacatoferrin-stimulated thymidine incorporation by comparing the effects of ironfree lactoferrin (apolactoferrin) with those of iron-saturated lactoferrin (diferric lactoferrin) under conditions that inhibit the transfer of iron between these iron-binding proteins in the bioassay system. In addition, we have compared the dose-response relationships of diferric lactoferrin and apolactoferrin. The results demonstrated that lactoferrin, independent of iron-binding states, promoted the incorporation of thymidine into the DNA of rat crypt enterocytes. These observations suggest a previously unreported nutritional role for lactoferrin that is independent of its iron-binding capacity.
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Nichols, B., Mckee, K. & Huebers, H. Iron Is Not Required in the Lactoferrin Stimulation of Thymidine Incorporation into the DNA of Rat Crypt Enterocytes. Pediatr Res 27, 525–528 (1990). https://doi.org/10.1203/00006450-199005000-00022
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DOI: https://doi.org/10.1203/00006450-199005000-00022
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