Abstract
Intereukin 18 (IL-1) is a mediator of many of the biological activities important in inflammation. It is still controversial whether there is increased activity of IL-1 in Crohn's disease (CD). Mucosal biopsies taken at colonoscopy from 5 children with CD and 5 control patients were studied. All children had presented with chronic diarrhoea, and in all 5 with CD a histological diagnosis had been made. 3 of the 5 were on immunosuppressive treatment with Prednisolone. The colonic histology was normal in 3 control children, but 2 had a mild non-specific colitis (NSC). 3 colonic biopsies were taken from each child, 2 was snap frozen and stored and 2 were cultured for 24 hours in 2 mls of standard culture medium. After 2 hours, LPS (50 μg/ml) was added to one of the biopsies. After 24 hours the biopsies and supernatants were havested and stored. An ELISA assay, with a sensitivity of 400-1000 μg/ml was used to measure the concentration of IL-1β in the supernatant. In supernatants from normal controls no IL-1B was detected but in 3/5 with CD it was present (0.05, 1.42, 1.7 μg/ml) and in 1 with NSC (1.1). Following stimulation with LPS, IL-1B was present in all with CD and 1 with NSC. In 3/5 with CD it rose markedly (0.2 - 0.8, 0.4 - 1.6, 0.5 - 1.4). In conclusion 1L-1B secretion was detected in CD and NSC. The phenomenon was enhanced by LPS and may not be specific to the inflammation of CD.
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Hill, S., Milla, P. & Mirakian, R. INTERLEUKIN 1B PRODUCTION BY COLONIC MUCOSA CHILDREN WITH CROHN'S DISEASE. Pediatr Res 26, 275 (1989). https://doi.org/10.1203/00006450-198909000-00072
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DOI: https://doi.org/10.1203/00006450-198909000-00072