Abstract
ABSTRACT: To determine the relation between milk intake and plasma levels of β-casomorphins as a function of age, we studied the levels of β-casomorphin-7 immunoreactive material (BCMIR) in 2- and 4-wk-old beagle neonates and adult dogs following milk intake. We used three kinds of milk: 1) bovine casein-based milk formula; 2) canine bitch's milk, and 3) soy protein-based (noncasein) formula. Using a new extraction technique, BCMIR was isolated from plasma and assayed by radioimmunoassay at 0, 2, 4, and 6 h after feeding. We found that BCMIR increased significantly in both 2- and 4-wk-old puppies post bovine and canine milk feeding, but not after soy protein formula. Base-line values were about 80-120 fmol/ml and increased to 100-120% 2 h after bovine casein intake in the older puppies and by 4 h in the younger group. BCMIR levels were undetectable in adult dogs before or after bovine milk feeding. β-casomorphin-7 added to plasma was rapidly degraded whereas BCMIR was stable. Chromatographic profiles demonstrated that the BCMIR moiety is larger than β-casomorphin-7. Our data suggest that the appearance of BCMIR in the plasma of neonatal dogs is a result of β-casein degradation along a permeable gastrointestinal mucosa. We speculate that morphiceptin and β-casomorphins can be cleaved from BCMIR and may have important physiologic effects.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Singh, M., Rosen, C., Chang, K. et al. Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs. Pediatr Res 26, 34–38 (1989). https://doi.org/10.1203/00006450-198907000-00011
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1203/00006450-198907000-00011
This article is cited by
-
Epigenetic effects of casein-derived opioid peptides in SH-SY5Y human neuroblastoma cells
Nutrition & Metabolism (2015)
-
A mini-review on health and nutritional aspects of cheese with a focus on bioactive peptides
Dairy Science & Technology (2012)
-
Further research for consideration in ‘the A2 milk case’
European Journal of Clinical Nutrition (2006)
-
Ausblicke in eine zukünftige Säuglingsernährung
Monatsschrift Kinderheilkunde (2003)
-
Autism and gastrointestinal symptoms
Current Gastroenterology Reports (2002)