Ontogeny of Epidermal Growth Factor Receptor/Kinase and of Lipocortin-1 in the Ovine Lung

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ABSTRACT: We have examined the ontogeny and distribution of the epidermal growth factor receptor/kinase (EGF receptor) and of lipocortin-1, a major cellular substrate of the EGF receptor, in a developmental series of 13 normal ovine fetal lungs (44-145 d of gestation) using the peroxidase anti-peroxidase technique with two extensively characterized polyclonal antibodies recognizing the EFG receptor and one polyclonal antibody recognizing lipocortin-1. Immunoreactive EGF receptor/kinase and lipocortin-1 were detected in conducting airway epithelium by the end of the first trimester of pregnancy before bronchial glands could be identified. This was followed at two-thirds of gestation by immunoreactivity in bronchial glands and large bronchioles adjacent to postive bronchi. By seveneighths of gestation conducting airway epithelium no longer contained consistently detectable immunostaining for EGF receptor, although lipocortin-1 was identified until term in all levels of conducting airways. In contrast, neither EGF receptor nor lipocortin-1 immunoreactivity was detected in alveolar type I or type II epithelial cells, fibrocytes, chondrocytes, smooth muscle, or endothelial cells at any gestational age. These findings suggest that EGF receptor and lipocortin-1 may participate in early airway development.

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Correspondence to Mildred T Stahlman.

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Johnson, M., Gray, M., Carpenter, G. et al. Ontogeny of Epidermal Growth Factor Receptor/Kinase and of Lipocortin-1 in the Ovine Lung. Pediatr Res 25, 535–541 (1989). https://doi.org/10.1203/00006450-198905000-00022

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