Abstract
Natural killer (NK) activity of peripheral blood lymphocytes (PBL) is reduced in GHD. We, therefore, investigated whether or not IGF-I and IGF-II have any influence on NK activity in the presence or absence of interleukin 2 (IL-2). PBL were obtained by fractionation of blood cells on a Ficoll gradient. After incubation in RPMI )640 medium with or without IL-2 (100 U/ml), IGF-I and/or IGF-II (25 ng/ml) for 15 h cytotoxicity was measured by a 4-h 51-Cr-release assay utilizing the cell line K562. IGF-I and IGF-II alone or in combination had no effect on NK activity. The stimulatory effect of IL-2 (100%) was not significantly enhanced by further addition of IGF-I ( 123 ± 48%) or IGF-II (118 ± 36%), if inter-individual means (N=11) were regarded, although in some individuals a significant potentiation (p<0.00l) was observed (maximal values with IGF-I: 232%, IGF-II: 194%). In contrast, addition of both IGF-I and -II potentiated the IL-2 effect significantly (p<0.001) even if the inter-individual mean was regarded (146 ± 125%). In 7 out of 11 individuals the combined effect of IGF-I and -II was clearly more than additive. Dose-response curves of IGF-I and -II in the presence of IL-2 exhibited a significant increase of NK activity between 0.1 and 10 ng/ml. It is concluded that (1) somatomedins are modulators of NK cell function and (2) that IGF-I and -II may act synergistically in particular systems.
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Blum, W., Handgretinger, R., Lingel, H. et al. 110 SOMATOMEDINS POTENTIATE INTERLEUKIN 2 INDUCED STIMULATION OF NATURAL KILLER CELL ACTIVITY. Pediatr Res 24, 535 (1988). https://doi.org/10.1203/00006450-198810000-00131
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DOI: https://doi.org/10.1203/00006450-198810000-00131
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